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Naturally, the first technique involved freezing the cells in order to retain living cells. For cells to remain stable, the ideal freezing temperature is roughly -80C. They are frozen for a maximum of three months, after which degeneration occurs.
The next step involves freezing the cells in liquid nitrogen at -196C to stop early cell deterioration.
However, there was a significant amount of risk, discomfort, and expense associated with the transportation and storage (logistics) of these frozen cells. From the production lab that may be located abroad, to the forwarding firm, the exporting country’s customs screening, the importing company, and finally, the clinics and hospitals. The aforementioned requires a significant
在到达最终用户之前，在运输或储存过程中的任何时候都不能将细胞从冷冻中取出或解冻。任何马虎或粗心的处理都会导致细胞严重降解或完全丧失功效。
